什么是FPV?111111电影

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出门在外也不愁[医][=feline panleukopenia virus]猫粒细胞缺乏症病毒
猫细小病毒;
大家都在背:
1. We also have constructed two shuttle vector plw 9 , psc 11 m 1 which can recombine to FPV genome.
我们还构建了可以和FPV基因组发生重组的穿梭载体, plw9和psc11.
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fpv的全称:
未分类的(4)
first pass ventriculography
首次通过法心室造影术
fowl pest virus
家禽疫病毒
fowl plague virus
家禽流感病毒
fixed-price vendor
固定价格卖主
fpt 首达时, fpv 猫瘟热, fpv 快相速度
- 基于1个网页
2. 猫细小病毒
fpv 猫瘟热, fpv 猫细小病毒, fpv 猫科动物
- 基于1个网页
3. 禽疫病毒
fpv 猫科动物, fpv 禽疫病毒, fpv 猫细小病毒
- 基于1个网页
FPV是英文First Person View的缩写,即“第一人称主视角”,它是一种基于遥控航空模型或者车辆模型上加装无线摄像头回传设备,在地面看屏幕操控模型的新玩法。 一般的遥控模型都是由操纵者手持遥控器,目视着远处的模型控制它的姿态,完成各种动作。但是怎样才能更加刺激和真实的体验呢?近几年随着电子设备的小型化,我们有机会把无线视频传输设备装在体积和载重量都非常有限的模型上,从而实现以第一视角操纵模型的方式。所能达
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fpv是什么意思
中文翻译飞行航径矢量家禽疫簿猫泛白细胞减少症病毒猫细小病毒首次通过法心室造影术细小病毒抗体
例句与用法1.Fpv flight path vector飞行航径矢量2.Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlierPuc18ha和sk质粒同时经hind 、 kpn酶切后连接得中间质粒skha ;将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点,通过酶切鉴定获得了pfgs11ha ;将含ndvf基因的质粒puc19f用hind 、 sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe / l启动子下获得中间质粒skf ,再将质粒skf和puc18质粒先分别用ecor 、 xho酶切klenow补平,后再共同用sac酶切连接得puc18pelf , sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位点,通过酶切鉴定获得了pe / l - f与ps - ha同向的表达载体pfgs11haf 。 3.In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv本研究采用脂质体转染方法,将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞,经6轮蚀斑克隆、筛选、表达, pcr鉴定和dot - elisa检测,证明该重组病毒已构建成功,并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。 4.Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538 , and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid . both vp3 gene and lacz gene were cloned into noti site of psy681 , recombinan fpv transfer vector containing vp3 gene of gpv was obtained . the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine本研究另从含有gpvh1分离株主要结构蛋白vp3基因的重组质粒pproexhtb - vp3中切取gpvh1株vp3基因片段,将其亚克隆于psy538的ecori位点,然后将带有痘苗病毒启动子p11的lacz报告基因平端克隆于上述重组子的smai位点,再用noti切下同时含有vp3基因和lacz报告基因的片段,再亚克隆于psy681的noti位点,构建出含有vp3基因的重组禽痘病毒转移载体,为构建表达vp3基因的重组禽痘病毒从而制备gpv基因工程疫苗奠定了基础。
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