求求虐英文翻译译

来源：蜘蛛抓取(WebSpider) 时间：2011-09-26 02:30 标签： 君子食无求饱翻译
求英文翻译Efficient (>90%) short hairpin RNA (shRNA)-mediated depletion of Otub1 did not result in 53BP1 foci formation (Supplementary Fig. 13a, b). By contrast, two independent shRNA constructs directed against Brcc3 (with knockdown efficiencies of 95% and 98%; Supplementary Fig. 13c, d) resulted in the accumulation of 53BP1 and ubiquitin chains at telomeres in cells expressing TRFcT (Fig. 4a and Supplementary Fig. 13e). Moreover, BRCC3 depletion in these cells resulted in levels of end-to-end chromosome fusions that are comparable to that observed in Trf2−/− cells (Supplementary Fig. 14). This latter result further corroborates the previous observation suggesting a critical role for 53BP1 localization for efficient DNA-repair events.To verify whether BRCC3-mediated suppression of the DDR is associated with the iDDR region of TRF2, we tested whether Trf2−/− cells expressing TRF1iDDR require BRCC3 expression to ensure end protection. Indeed, shRNA-mediated downregulation of Brcc3 in these cells abolishes the protective role of TRF1iDDR, resulting in levels of 53BP1 localization and chromosome fusions that are comparable to the ones observed in Trf2−/− cells (Fig. 4a–c).To identify proteins that can be recruited at telomeres by the iDDR region of TRF2, Flag-tagged TRF1iDDR was immunopurified and analysed by mass spectrometry (Supplementary Fig. 17 and Supplementary Table 3). As a negative control, we used Flag–TRF1.
温少来袭199258
高效率（> 90％）的短发夹RNA（shRNA）的介导的枯竭OTUB1并未导致53BP1灶形成（补充图13A,B）.与此相反,两个独立的shRNA的构建针对BRCC3（拦截效率为95％,98％,补充图.导致53BP1和泛素链在细胞中的端粒表达TRFcT的积累（图4a和补充图13c中,四） 13e的）.此外,在这些细胞中的BRCC3耗尽导致水平的终端到终端的染色体融合,与观察到TRF2 / - 细胞（补充图14）.后者的结果进一步证实了先前的观察,提出了至关重要的作用53BP1本地化高效的DNA修复事件.为了验证是否解除武装,复员和重返社会的BRCC3介导的抑制是与的IDDR区域TRF2,我们测试TRF2 / - 细胞是否表达TRF1iDDR需要BRCC3表达以确保最终的保障.事实上,在这些细胞介导的shRNA的表达下调BRCC3废除的TRF1iDDR的保护作用,从而在水平的的53BP1定位和TRF2 /观察到的具有可比性的染色体融合 - 细胞（图4a-C）.IDDR TRF2的地区,可以招募端粒识别蛋白质,Flag标签TRF1iDDR的免疫纯化和分析由质谱（补充图17和补充表3）.作为阴性对照,我们使用TRF1旗.
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