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可耐受的最高摄量条件下补硒对机体代谢的影响.pdf110页
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可耐受的最高摄量条件下补硒对机体代谢的影响
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华中科技大学博士学位论文 摘 要
Reference Intakes 标准，其制定依据是根据临床观察指标 如指甲有无变形或者脱发 Intake
推断硒的可耐受的最高摄入量 UL，Tolerable Se／d，并指 Upper Level 为400“g 出服用此剂量不会出现毒副作用。我们认为制定UL的依据比较粗糙，缺乏微观的 、卜“～7。
实验证据。因删本文设计了动物实验和人群实验研究在uL条件下补硒对机体代谢 ／
的影响，并探讨了相应条件下分子生物标志物的存在。研究结果可以为补硒研究提
供一定的依据。主要内容包括： 1．分别采用亚硒酸钠和硒酵母为补硒源，利用高分辨的液体1H．NMR研究在
UL条件下补硒对大鼠尿液代谢产物的影响，同时采用组织病理学进行验证。／结果
表明：在此条件下，尿液中已经观察到硒对大鼠的肝脏和l肾脏损伤的公认的生物标
志物，同时发现在此条件下大鼠尿液中检测出了甲酸盐，此结果与硒的ROS毒性机
理相符合，我们认为可以把甲酸盐作为硒中毒的NMR标志物，为进一步研究硒的
uL值提供参考。组织病理学检查结果也表明在此条件下，肝、肾组织已经发生病
变。 。户7―一
的粮食硒含量和生活用水中硒含量分别为普通地区的几十倍，而高硒区人的尿硒和
发硒含量分别为普通地区正常人的8．6倍和6．4倍。屎用杨光圻的经验公式推算『F常
地区人和高硒区人膳食硒摄入量分别为133．0+35．5，0．5 pg／d，后者为前
者的8．5倍左右，该结果与杨光祈等提出得UL值无显著性差异。采用1H．NMR分
析高硒区人尿液和普通地区正常人尿液，结果发现基本上与动物实验的结果一致，
但同时观察到另一个与肝脏的毒性损伤相联系的分子标志物牛磺酸的出现，浚结果
可以反映硒在
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KG&& &Type:93/G2-120 5.6KW??L??1200siemens&& &DZ47LE-63 2P C6Baumer&& &HEAG151,5mA-5Vhydac&& &DF BN/HC 160 G 10 D1.Xpma&& &KS800-01Heidenhain&& &SKF&& &SKF 177-300-114&Leine&Linde&& &466.33-10-10Rexroth&& &Z2S6-30/Bhydac&& &KHM-40-F6-11141-06X with flange /welded joints and sealTapeswitch&& &Type:TS-6, color:Black , length=540mm ,4-wireRexroth&& &Rollomatic Deutschland&& &124953SKF&& &PILLOW BLOCK HOUSING | SKF | SNL 532 |banner&& &Q12AB6FF15REXROTH&& &ZDR6DA2-4X/75YSTAHL&& &-AREltrotec&& &AFV-C0946S (9.133.4DTRAFAG&& &NAT250 7ABB&& &PFCL201CE-50KNSiemens&& &7MA2000-8ABWOERTZ&& &4030S 70m?O 500V,192ARexroth&& &4WE6G73A62/EG24N9K4/A12END-Armaturen&& &10.029CRANE&& &VALVE/AMGD5C1MZH_CRANEFLOW;DN100-PN10;RAL9006rexroth&& &Pilot operated check valve SL10PA1-4XPOWER-GEMS&& &EB480PR/POWER-GEMSUnion Instruments GmbH&& &UTS- UTS-&UAS&& &230??240V 0.6A 50/60HZStaubli&& &RBE03.1904/IC/JETR-Electronic && &CE 100m 100-05128STAHL&& &LAMP HOLDER/6041/42&parker&& &11C78-25-12siemens&& &ET220U??6ES5 318-8MB13Schmersal&& &T2A067-10/10YVOGEL&& &MKU1-BW6-SPF2&& &ZK28 92SHABostonGear&& &FWAG&hydac&& &HDA3840-A-400-124siemens&& &DK-8&Datalogic&& &JOHNSON PUMP&& &SRZS 441 W G11SVETRUCK&& &902159TOX&& &S 15.100.12Dparker&& &G38ZSCFWestlock&& &-SIEMENS&& &1FT-2AG1SFERAX GmbH&& &XA 815Rexroth&& &R FESE 50 CA-3X/MPMA&& &KS40-108-909M-D35parker&& &4D-C1GODRexroth&& &HM17-11/315-C/V0/0SEIM&& &PX083#3BOROHBX01X0300GMN&& &S607CTAP4DULtrafag&& &S/N: 1HZC2 P1abs:0.62MP@20?? P2abs:0.60MP@20??motor&& &1LA 1.1KWrexroth&& &ABZMS-36-1X/0500R-K24&SKF&& &DU56N4125368SULZER&& &Part No.:29 D 35/20??5 MAT.:HGW (DRAWING No.:8264476)SGM&& &26/24-20,5 Shore 90 Apolycilp&& &162265WITTENSTEIN alpha GmbH&& &HTB-30L34C10-30F1024B-S8-C10&REXROTH&& &4WRPEH 6 C5 B24L-2X/G24K0/F1Mmoog&& &D661Z2725H&psysteme&& &PSY-LA-20L,Hydac&& &DFBN/HC660TF10C1.0(311971)HEIDENHAIN&& &ERN180 -03 IDEsiemens&& &6ED-0BA0&Rosler&& &??RDT?C150/S5 Nr:43152/08??&woehner&& &31110PARKER&& &Solenoid Valve KReinshagen&& &15.13.400 SIEMENS&& &1PL-0EB0leine&Linde&& &861-4Rexroth&& &4WRZ25E325-70/EG24N9K4/M&RSP&& &Spring _NW48Gessmann&& &V64 D-3??-1Zehnder Comfosystems&& &M35 91744 CLF 230V-50/60HZ 240WRexroth&& &Z2FS6-40/2Q&REXROTH&& &250 LDN 0100 H10XL-B00-00V5,0-R0M0AEKEYENCE&& &KEYENCE-OP-84394ATB&& &ENF63/2C-11/,24ATB&& &CAF90L/2D-12 200V 3KWSchlatter Industries AG && &90308RTK&& &RSM63/8FG&& &IA10/MOW3-40VOGEL&& &MFE5-5068+1FWheidenhain&& &ROD220/9000??Id.Renold && &A2060X10FTMAINA&& &C_A074_KC52_13_DLZ001??MAINA TYPE BC28)STAHLWILLE&& &stoeber&& &FOS4040/B 4.0KVA&Rexroth&& & 24v 0.83A&Roemheld GmbH&& &KTSGMVDCweidmuller&& &DRM270024LTRohde & Schwarz Vertriebs-GmbH&& &R/S FSP13+softwareSIEMENS&& &6ZYSIEMENS&& &SITRANS F M MAGFLO MAG 1100Telemecanique && &XS1-N18PA349Dsiemens&& &IC693MDL660SAMSOMATIC&& &Inspection glass(for A.)Roblon&& &ID.2048 12FEB2007ALLEN BRADLEY&& &1756-CNBRSiemens&& &6ES7 151-1BA02-0AB0POMINI && &/800-130-004-01 The grinders number: S-Nr.: 555Euchner&& &83965Rexroth&& &DR10-5-5X/200YMSMW && &193144NCFLENDER&& &20HTF30AP001 No0-001SIPOS&& &2SYROFA && &WICHE KB 240RAKO&Siemens&& &6SG-Z+CBP2Herzog&& &HGT915supfina&& &20*17*1000/NI. STAHL&& &CMP -11Trumpf&& &566395Tecnolubeseal && &PS.50bar,PT.75bar,SOUPAPE TYPE.S178WOMA&& &200.1556LAMBDA&& &ZWQ80-5225/Aparker&& &SCP-250-24-07&SMW&& &18345ALCO Controls&& &PS3-W6S PCN:0715556&THERMOFISHER && &ICP spectrometer dedicated spray chamber number: ARIPAX&& &TMpolycilp&& &157095Rexroth&& &ZDR6DP-2-4X/75YMVWITTENSTEIN alpha&& &SP180S-MF1-10-0K1 2571280 SET 1 ALPHA DE NTelefonnummern&& &M10*8.5 12.9??Monty 3300??TUNKERS&& &V2 63.1 A10 T12 130??&& &DC2C- Serial 067047 DC 0048 AC120V&&& &skiip 39AC12T4V1RITTAL&& &622775RAVARINI CASTOLDI & C&& &E99.021014REINEKE&& &o-ring ??55*2.5|Viton|48/41089 4HP-72-1968tool-temp&& &C-570/571/572 (Z type pump without motor)Siemens&& &6SL-0AA0siemens&& &A5EA, Pos. 2heidenhain&& &ID:TRACO&& &TCL 060-124 60W&REBS&& &SV-G1/2A-0 NO:9818056ROFIN&& &Putzmeister&& &527853TOX PRESSOTECHNIK GMBH & CO. KG&& &Id-Nr 167282Parker&& &07E46B11ACStaubli&& &MPX06.7101/JVMoeller&& &NZM2-XR208-240VACEmhart Teknologies&& &Z 142 046/6HAWE&& &52 H 63/63C 70X/EAGMN&& &00 1/MM 100-167-200HZ 188-315-380V 0.65-1.1-1.1KW S1-100% 4.2A&STAHL&& &-093-001Rexroth&& &4WRZE16W8-150-7X/6EG24K31/A1D3MSTOEBER&& &FBS4013/BWERMA&& &woerner&& &KFI-E/B/W/W400/100 NO.086538&& &valve plate for SC-064Rspiraxsarco&& &DN20 PN16 BRV73rexroth&& &R L=1886mmpolycilp&& &174282tuenkers&& &SZKD 40.1 T12parker&& &P70CF-01-HK28-002ELAU&& &SMS1B1POLO Filervlies&& &POLO Filervlies/Polo Type 370SIEMENS && &3VF-0AA0&ROCLA && &LY-DESRM0158Telemecanique&& &LC1D 150 24Vheidenhain&& &hydac&& & W/HCC/FBSIEMENS&& &3RTRexroth&& &RWRZE 25 W6-325-7X/6EG24N9K31/F1D3M&SAIA&& &NR. XCG5-81-J4 MICROSWITCH WITH LEVER 35mm 250VASKF&& &ANGULAR CONTACT BALL BEARING | SKF | 7320 | BECBP | 100 X 215 X 47 |Weidmuller&& &IES21-SW6/2SCS 461.126.17.AM lechlerRexroth&& &4WRPEH10CB50L-2X/G24K0/F1M ROSS&& &G3/4 A Dhydac&& &0160 D 005 BN4HC\-VAmetek&& &SAMETEK && &STS100A-250&ROSS&& &D654K01/ISO Gr.4(.404)SIHI && &USE FOR ZHND040200-AA-BJ3-1B-2&SIEMENS&& &3NPTHIEM&& &,DKF 80NR KWSTAFFA&& &rittal&& &SKParker&& &T6ED-066-045-4L02-B1diatest&& &NHM2-270&WOERNER&& &typ GMZ-E no.SCHMALZ&& &P/N: 20.03.01.00003 BE-35-E-S-Dballuff&& &BES516--D-R05SIEMENS&& &3RTReckmann&& &1R15-407-160-12-2402leine&linde&& &00siemens&& &3VU 5-8TERADYNE DIAGNOSTIC&& &NGVI UNIT MAA31/I|P Voltage 12ROFIN-LASAGLASERS&& & ballast resistors for 85kVAALLEN BRADLEY&& &700CF220DJRexroth&& &4WRSE6V04-3X/G24KO/A1V-280SIEMENS&& &3RTRittal&& &SZ2514000SARTORIUS&& &Part No.43 0.2??&TOX&& &S8.30.100.12DTippkemper-Matrix GmbH&& &UDC-S24-A&ROCKWELL&& &1769-OB16SIEMENS&& &1LG-ZAEG&& &2A400-350HFRL1SEDIS&& &MAILLON JONCTION POUR 5810-01Setra&& &SRH13PD2CT2NLEUZE&& &SCANNER BCL 34R1 F 100Rexroth&& &4WE 6D6X/EG24N9K4&& &SN 401-2-AKRTK&& &ST MXEBURexroth&& &FRM16-32/100LGestra&& &GK 11;DN=150;PN=1.6microsonic&& &DBK-4/EMPF/CEE/01M8Wollschlaeger&& &5981 4TKSYSTEM ENGINEERING && &VALVE/RHD16SOMDCF??B.&REXROTH&& &3WE 6 B62/EG24N9K4hydac&& &DBT-DV-08-01X/0&wiha&& &260P 004866PARKER&& &FM3DDDSV&SOCOMEC&& &/RMS 100 3P/Fuse-switch, 3pol., 1 Aux.Contact, 22x58 FuseTelco&& &LT110LTS3815SKF&& &DS-W18-4-LPRUSS && &L179 DN15 PN40UNIVERSAL HYDRAULIK&& &EKM-518-T-CN-WSW G3/4 Ari-Nr??parker&& &E2B040Z300NMK3Spandau Pumpen&& &PUMPE PXAAA 1.5KW;230-480;50/60HZ +Arittal&& &SKABB&& &ST4.1.1.1.1.50.D.A.1.5.A.2.E.O.A.B.A.1SCHILEICHER && &SN01005-24parker&& &GE16SREDOMDCFWeir&& &SMLC 50R 1000PEPPERL+FUCHS&& &NJ1. 5-6. 5-40-E2-V3&& &0.02mm&KRACHT&& &VC0.4 F4PS F-11SWAC&& &MC2 106-O-40A0MANN+HUMMEL GMBH&& &WK950/3PHOENIX&& &QUINT-PS-100-240AC/24DC/40&rexroth&& &RPTS && &|CHAIN|12A-1 18 Festival&WILLIELBE GELENKWELLEN GMBH&CO.KG&& &Sliding wheels/ 100.110GR.01 hole 20H7 upPanasonic&& &DPH-L100Richter Elektrotechni&& &CR16-150/020/09&SCHNEIDER&& &NO:32803moog&& &D661-4055/P80HABF7VSX2AALPHA && &LP 090-M01-7-111&KRACHT&& &KF25RF4-D15 with coupling,hood and motor 380V/1.5KW B5 class 4R.STAHL&& &8003/5NA-016-3SUN&& &1A82-AAHEIDENHAIN&& &F ROD486 1000Gestra&& &RK44 DN100 (4??)PN6-16 SF20 708717P+F&& &V1-G-PG9pmc&& &PT100-N-HA-Sschneeberger&& &NK2-65ultrafilter&& &SMFP15/30Hydac&& &VST-18X1.5WD&& &186.12.1.1.50.1S+024.91.001&STAHLWILLE&& &730DRSIPOS&& &26.600-6001-R-1-100-300-1-000, 0-6BAR,4-20MA,12-360DC&KRACHT&& &PUMP/KF6RG10-MSA60/8 PUAG2629&& &SNV 4063KLWolfgang Warmbier&& &METRISO 2000-ESD TEST InstrumentWoodward SEG GmbH & Co. KG&& &XU1-ETUNKERS&& &V2 50.1 A10 T12 90??ABB&& &DSDX453LSPM&& &ALMR2-TP43A-MK12.7-L1000-SV L1=150HAZET&& &803-55SCHUNK&& &OSE-B22-354210Rexroth&& &4WE6D6X/EG220NZ4ABB&& &M3ARF 100LB4 3GAR102402-BSE 3KW,400V,1430R/MINsiemens&& &6SN-0CA1 36/47KW&rexroth&& &DBDS30K-1X/200SCHUNK&& &GZB-S,??12MM~??8MMpulsotronic&& &ID strapex&& &PN.&FACOM&& &R.300B&turck&& &BRM42-R-VP6XKEYENCE&& &SENSOR|GT2-CH2Mparker&& &PAV10RKP4DREXROTH&& &CDM1MT4/40/22/490A21/B11CGDMWWINA&& &KR47-PPABB&& &4??F/400V R4E310 AF12 PUHALTIMELLE 5??F/400V ABBABB&& &LB 217-011/001 200KNSchonbuch&& &LLDE 0504 A67grundfos&& &CR3-3 A-A-A-E-HQQEMURR&& &3P480VAC/3P 220VAC 1.5KVAhawo GmbH && &ht Seal Check HDPEHEIDENHAIN&& &ID:Renishaw&& &no type and see the picturesiemens&& &6ALsiemens&& &VKF46.200+ASK46.4ZF Friedrichshafen AG&& &EKER-8/PULSAR&& &PTR2000 BV40-121-1STAHLWILLE&& &735 40LEGRAND&& &4873Rexroth&& &A10VSO18DFR/31R-PPA12N00;R&pomini&& &-7Pister&& &BKII-G1/4"PN-500 DN-16Rexroth&& &WE 6 J62/EG24N9EK4/B10ROLAND&& &A70P350-4SCHUNK&& &PZN+ 100/2AS 303612&& &IF10-30L-10/01Pparker&& &1CA43-22-10Magnet-Schultz&& &2281546, AWEX 020 D06 Speisung18-36VDC messweg??20mmwatt&& &ASA 75A 134M4 KTY84 BR60 IG ER58FP15R-H4PR-0WALTHER-PRAEZISION&& &see the picture Oil line quick-change connectors (big)schroff&& &TIPPKEMPER&& &IRL-201-S/E-S39 Emitter / ReceiverStaubli&& &RBE06.7101/JE&Zimmer&& &DNR98-3/12-AVZross&& &18643RWhiteOak&& &C1V-12a 1200mA(with a 3 m wire)Heidenhain&& &DS EUROPE && &LT1-A1-200Kg&VIPA&& &323-1BL00&BIAX&& &SARH 820schunk&& &219224 SDF-WZS SK 50 ? 12&TKSYSTEM ENGINEERING && &GASKET/GDM3-??B.&PFEIFFER&& &BN846469-TSTAEUBLI&& &Connector (male) /MALECOUPLINGSPM12/SPM125433/IA/JV??B245.925.04REXROTH&& &CY210G150/90-XHKM11A CylinderStaubli&& &RBE03.7251/ICpolycilp&& &21564heidenhain&& &HEIDENHAIN&& &STAHL&& &-075-10THOMAS ELEKTROWAERME GMBH&& &Rohrheizkoerper 860 500W 230VSera&& &FLP2,9009396&PSG && &ETS 132net 32FZ TC/PT TC/PT NO.040NIEDAX&& &Cable trays 100x35mm galvanized steel without cover including hanging and assembly, RL35.100 ,L= 66M&heidenhain&& &REXROTH&& &Valve 4WE10J3X/CG24N9K4Linos&& &Rod 75 mm G06 &SCHNEIDER&& &C60NC41APstricker&& &S-1250-GS-22-GI-SF,black,siliconefree,steel-cable,thickness:9,5+/-1mm,breadth:210+/-3mm,total Length:231.6mHANNING && &OE6E082B-398 220-230V 50HZ 0.3-0.5A /MIN (with fan blade)Heidenhain&& &ERN480 -03 ID:385 480-37PARKER&& &HNVSYY36S/CH??6mmSTAHLWILLE&& &zitec&& &291582,INTRASTAT?NR.: VOGEL&& &MPV01 / 3.0&Ant Paar&& &DMA35 Ex Petrol Measuring range:1.0-1.4Siemens&& &6SL-6AA3parker&& &481-06HEIDENHAIN&& &SKF&& &PCM8085100ETechnor Italsmea S.p.A.&& &CODICE:S/400 PA-230 REAT TORE PER LAMP NA-AP 400W 220-240VMANNFILTER&& &TYPE: WD13145/17SIDO&& &10/parker&& &ET12SOMDCFreichle&de-massari ag&& &5990rexroth&& &M-SR15KE02-1Xhydac&& &HCTelemecanique&& &RXL3A10B2P7/230V 50/60HZrs && &TA32,1000/1AZIMMER&& &MBPS3005AS1rexroth&& &R S 20 A1.0RATHGEBER&& &ETDLTVA 500-400/230VWeishaupt&& &W-DK3/01 S-2SKF&& &MKU1-BW6-2F003JWarmbier&& &.10abb&& &ACS800-01-1Silicon Software&& &SSD-C08G-4500&parker&& &EW12ZSCFrexroth && &4WE6G62/EG24N9K4AEG&& &MCE 409S 3400 IrA 400ASCHMITT&& &SB-4120-LRexroth&& &3DR10P5-6X/200Y/00MRexroth&& &LC50A40EXREXNORD&& &SSC8811TAB-K325 L= 19.5MPawkywonne Controls GmbH&& &Art:NF532230ACheidenhain&& &559 302-01Rexroth&& &RWREE6V1-08-22/G24K31/F1V&& &SN 401-3-TDIhydac&& &4WE 10 D33/QFCW230N9K4roeckelein&& &XT-ZS 05813 purple 150 25 8 S extra coarseS+S REGELTECHNIK && &THERMASGARD ETM4-I 29-900 MB ;TEMPERATUR :EINSTELLBAR UB=15-35V DC AUSGANG:4-20 PMAX=40BAR KW 40/2010&Process-Electronic && &CS87-L700 1 Inch&SAT&& &8 LECA 9 50?? 8 LECA 9 CT PP50-500-000-ROV00 Anschlu?:3/4"Betriebsdruck:8bar Inkl.Filterkerze:38.N9 CT PP50 S00-0V Porenweite: 50??,Rexroth&& &DBDS6K1X/100E RProcess-Electronic&& &P/N C1796 SN.313494&& &MY4IN 24VDC&Schneider&& &XKM-B121210rexroth&& &SCA25BZ2X/MPRO-HUB&& &RICKMEIER && &R25/10 FL-Z-DB&VEGA && &S83FZO.ZZVM040X 400mm2"150Lb AB-NR??&Bosch Rexroth&& &SCHMACHTL && &POSITION / DISTANCE SENSOR | LE
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