Phase Critical Assemblies
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The dielectrics and constructions&used in our phase critical product line have been developed to optimize the phase performance of the finished assembly.
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&with Triple Layer Shield and TF4 Technology
&Semi-Rigid with&TF4 Phase Performance
&TFlex type cables with TF4&Phase Performance
&Semi Rigid for ultimate Phase Tracking Performance
&for Low Smoke / Low Toxicity Applications
For assistance in choosing a product or the latest information on Phase Critical Products, contact your
For a comparison and overview of the&evolution of these&technologies, see our published article ,
&&&&& and&&
To download a copy of the&. &Solid-phase cloning for high-throughput assembly of single and multiple DNA parts.
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2015 Apr 20;43(7):e49. doi: 10.1093/nar/gkv036. Epub
2015 Jan 23.Solid-phase cloning for high-throughput assembly of single and multiple DNA parts.1, 1, 1, 1, 2, 1, 1, 3, 4.1KTH-Royal Institute of Technology, School of Biotechnology, AlbaNova University Center, Stockholm 10691, Sweden.2KTH - Royal Institute of Technology, Science for Life Laboratory, Stockholm 17165, Sweden.3KTH-Royal Institute of Technology, School of Biotechnology, AlbaNova University Center, Stockholm 10691, Sweden KTH - Royal Institute of Technology, Science for Life Laboratory, Stockholm 17165, Sweden Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2970 H?rsholm, Denmark.4KTH-Royal Institute of Technology, School of Biotechnology, AlbaNova University Center, Stockholm 10691, Sweden johanr@biotech.kth.se.AbstractWe describe solid-phase cloning (SPC) for high-throughput assembly of expression plasmids. Our method allows PCR products to be put directly into a liquid handler for capture and purification using paramagnetic streptavidin beads and conversion into constructs by subsequent cloning reactions. We present a robust automated protocol for restriction enzyme based SPC and its performance for the cloning of &60 000 unique human gene fragments into expression vectors. In addition, we report on SPC-based single-strand assembly for applications where exact control of the sequence between fragments is needed or where multiple inserts are to be assembled. In this approach, the solid support allows for head-to-tail assembly of DNA fragments based on hybridization and polymerase fill-in. The usefulness of head-to-tail SPC was demonstrated by assembly of &150 constructs with up to four DNA parts at an average success rate above 80%. We report on several applications for SPC and we suggest it to be particularly suitable for high-throughput efforts using laboratory workstations. (C) The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.PMID:
[PubMed - indexed for MEDLINE] Schematic outline of solid-phase cloning. (A) RE-based: biotinylated PCR products are captured and purified on streptavidin-coated paramagnetic beads (1). Solid-phase digestion of immobilized DNA, followed by washing away of cut pieces and buffer exchange (2). A second restriction enzyme cleaves at a site close to the beads (3). The supernatant is separated from the beads and the restriction enzyme is heat-inactivated. This purified and cleaved PCR product is ligated into a vector (4). (B) Head-to-tail assembly: PCR amplified backbone and inserts are separately immobilized onto streptavidin-coated paramagnetic beads. (1) Non-biotinylated strands are eluted in NaOH. Eluted backbone strands are discarded while the eluted insert strands are kept. (2) The eluted insert strand hybridizes to complementary sequence on the bead-bound fragments. (3) A polymerase is added to extend the two strands. (4) To introduce more inserts, steps 1–3 are repeated. (5) The final construct is released from the beads by one restriction enzyme cutting at both ends, allowing it to be ligated and circularized.Nucleic Acids Res. 2015 April 20;43(7):e49-e49.Results from restriction enzyme-based SPC. (A) Out of 60 735 cloning experiment, 51 556 were successful and 9173 unsuccessful. (B) The length of inserts did not have a clear impact on the success rate although a slight decline could be perceived for the longer inserts. (C) High GC-content of inserts was clearly affecting the overall cloning success rate negatively. The success rate dropped from above 90 down to 10% as the GC content increased from 55 to 80%. (D) Similar decline in success rate was also observed for sequences with LC-values below 0.67.Nucleic Acids Res. 2015 April 20;43(7):e49-e49.Success rate of RE-based experiments (blue bars, 1–46) grouped by insert characteristics (squares). The colored boxes indicate the magnitude of linguistic complexity content (green-red) or GC-content (yellow-blue) of inserts. Inserts with a low GC-content were unambiguously more successful compared to inserts with a high percentage of GC.Nucleic Acids Res. 2015 April 20;43(7):e49-e49.Unit-operations for RE-based and head-to-tail SPC. The light gray boxes indicate operations performed inside and yellow boxes indicate operations performed outside liquid handlers. Pink boxes are operations requiring temperature blocks while blue boxes indicate operations carried out in room temperature. (A) Restriction enzyme based SPC: PCR amplified inserts are automatically purified digested and subsequently ligated into a predigested vector. (B) Head-to-tail SPC: PCR amplified inserts and backbones are automatically purified, sequentially assembled by overlap extension and circularized into a plasmid. Transformation of Escherichia coli is done outside of the robot for both protocols.Nucleic Acids Res. 2015 April 20;43(7):e49-e49.Results from head-to-tail SPC. (A) Comparison of compatibility of thermostable polymerases with the head-to-tail method. The polymerases with best proof-reading capabilities, Phusion and Deep Vent, all failed to generate transformants with the standard head-to-tail protocol. (B) Activity was retained for protocols using Phusion after supplementation of the washing buffer with SDS. (C) Colony screens of inserted region representing a selection of assemblies of various lengths and number of inserts assembled by head-to-tail SPC. Final construct sizes spanned 2.9 to 7.2 kbps.Nucleic Acids Res. 2015 April 20;43(7):e49-e49.Publication TypesMeSH TermsSubstancesFull Text Sources
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fref是什么意思 fref在线翻译 fref什么意思 fref的意思 fref的翻译 fref的解释 fref的发音 fref的同义词 fref的反义词 fref的例句 fref的相关词组
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freffref 网络解释1. abbr. fixed 固定利率出口融资fref 网络例句1. As examples, consider calls to the functions fobj and fref. & &例如，考虑对函数 fobj 和 fref 的调用。2. Based on the proposed harmonic-locked technique, the divided frequency fdiv will lock with m times the frequency of fref. & &藉由提出的谐波锁定技术，除频讯号会锁在参考频率的m倍。3. In the call to fref, the parameter type is a reference to const. Conversion to const for a reference parameter is one of the acceptable conversions, so this call is also okay. & &在 fref 的调用中，形参类型是 const 引用，对引用形参而言，转换为 const 是可以接受的转换，所以这个调用也正确。4. The fobj function copies its parameters, whereas fref's parameters are references & &fobj 函数复制它的形参，而 fref 的形参是引用5. The call to fref, however, is illegal. When the parameter is a reference ( Section 7.2.4, p. 240), the arrays are not converted to pointers. The types of a and b don't match, so the call is in error. & &但是， fref 的调用是非法的，当形参为引用时（第 7.2.4 节），数组不能转换为指针， a 和 b 的类型不匹配，所以调用将出错。6. Stitch hanging lantern outdoor counted cross fref pattern stitch bear care cross stitch ... & &这句话挂花灯户外算过fref格局缝紧照顾交叉缝合。。。7. In this paper, the design of a spectrum of low-frequency low-noise removal circuit, Circuit by the low-noise preamplifier, reference signal source, switch-mode phase-sensitive detector and fixed-frequency components of narrow-band-pass filter, After assembly, debugging and testing, or to short-circuit input noise voltage of ,Fref set up as a result of the range of 1 ~ 9999Hz, stepping to 1Hz, the frequency by changing the Fref different frequencies can be achieved in weak signal detection. & &本文设计了一款低频低噪声频谱搬移电路，电路由低噪声前置放大器、基准信号源、开关式相敏检波器及固定频率的窄带通滤波器组成，经组装、调试与测试，折合到输入端短路时的噪声电压为，由于Fref设置范围为1~9999Hz，步进为1Hz，通过改变Fref的频率就可实现对不同频率的微弱信号的检测。fref是什么意思,fref在线翻译,fref什么意思,fref的意思,fref的翻译,fref的解释,fref的发音,fref的同义词,fref的反义词,fref的例句,fref的相关词组,fref意思是什么,fref怎么翻译,单词fref是什么意思常用英语教材考试英语单词大全 (7本教材)
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&2016 　京ICP备号-6　京公网安备30　记住，运行任何一个阶段的时候，它前面的所有阶段都会被运行，这也就是为什么我们运行mvn install 的时候，代码会被编译，测试，打包。 Maven的插件机制是完全依赖Maven的生命周期的。
Furthermore, a build phase can also have zero or more goals bound to it. If a build phase has no goals bound to it, that build phase will not execute.
此外,每个build阶段可以有0到多个目标与其绑定。如果一个build阶段没有目标与其绑定，那这个build阶段不会执行。
Maven有一个概念：goal。每个build阶段可能包含一个或多个。
The first, and most common way, is to set the packaging for your project via the equally named POM element &packaging&. Some of the valid packaging values are jar, war, ear and pom. If no packaging value has been specified, it will default to jar. Each packaging contains a list of goals to bind to a particular phase. For example, the jar packaging will bind the following goals to build phases of the default lifecycle.
在packaging标签中，你所选择打成什么样的包，会自动将一些goal绑定到相应的build阶段。默认是jar。
&modelVersion&4.0.0&/modelVersion&
&groupId&com.a.b&/groupId&
&artifactId&test&/artifactId&
&packaging&jar&/packaging&
&version&1.0-SNAPSHOT&/version&
&name&testI&/name&
&url&http://maven.apache.org&/url&
要想将写的程序和它本身所依赖的jar包一起build到一个包里，使用maven-assembly-plugin是一种选择，简单的结构如下：
&project xmlns="http://maven.apache.org/POM/4.0.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
xsi:schemaLocation="http://maven.apache.org/POM/4.0.0 http://maven.apache.org/maven-v4_0_0.xsd"&
&modelVersion&4.0.0&/modelVersion&
&groupId&com.a.b&/groupId&
&artifactId&test&/artifactId&
&packaging&jar&/packaging&
&version&1.0-SNAPSHOT&/version&
&name&testI&/name&
&url&http://maven.apache.org&/url&
&groupId&org.apache.maven.plugins&/groupId&
&artifactId&maven-compiler-plugin&/artifactId&
&version&2.3.2&/version&
&configuration&
&source&1.6&/source&
&target&1.6&/target&
&encoding&UTF-8&/encoding&
&/configuration&
&artifactId&maven-assembly-plugin&/artifactId&
&configuration&
&descriptorRefs&
&descriptorRef&jar-with-dependencies&/descriptorRef&
&/descriptorRefs&
&/configuration&
&executions&
&execution&
&id&make-assembly&/id&
&phase&package&/phase&
&goal&attached&/goal&
&/execution&
&/executions&
&/plugins&
&dependencies&
&dependency&
&groupId&org.springframework&/groupId&
&artifactId&spring&/artifactId&
&version&2.5.1&/version&
&/dependency&
&/dependency&
&/dependencies&
&/project&
但是要注意一点：
The main goal in the assembly plugin is the single goal. It is used to create all assemblies. All other goals are deprecated and will be removed in a future release.
官网说，下面的goal已经过时了（虽然deprecated，但是maven3.0.3还是可以使用的，只是可能以后更新maven的时候，这个插件不会更新了，这可能更新了maven，这个插件的这个goal不好用了！）
&goal&attached&/goal&
只要使用下面的替换上面的配置就可以了。
&goal&single&/goal&
下面的参考网址:
如果想要得到比assembly plugin 更多的控制，请使用Maven Shade Plugin
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