Pregled bibliografske jedinice broj: 245960
Pavi?, V Mio?, B Su?i?, V Bara?, Z Vnu?ec, I Prpi?, Z ?okljat, Zvonko
Vanj?tina creske ovce( Exterior characteristics of Cres sheep )
Sto?arstvo () 60 (2006), 1; 3-11
Vrsta rada:
Klju?ne rije?i:
C Vanj? K Tjelesne mjere( C body measurements )
U sklopu projekta kojemu je cilj utvr?ivanje vanj?tine i genetsko definiranje hrvatskih izvornih pasmina ovaca provedeno je komisijsko ocjenjivanje ovaca na tri obiteljska gospodarstva i jednoj poljoprivrednoj zadruzi na otoku Cresu. Ocjenjivanje creskih ovaca se sastojalo od subjektivnih metoda procjene vanj?tine, mjerenja ovaca Lydtinovim ?tapom i vrpcom te pojedina?nih vaganja. Izvr?ene su izmjere ukupno 350 grla, od toga 63 ?ilje?ice, prosje?ne dobi od oko 14 mjeseci, 66 mladih ovaca u dobi izme?u 2 i 3, 5 godine, 206 odraslih ovaca starijih od 3, 5 godine i 15 odraslih ovnova. Tako?er su uzeti uzorci krvi od ?etrdesetak grla koja nisu bila u srodstvu kako bi se mogle izvr?iti genetske analize. Prosje?na visina grebena odraslih ovaca iznosila je 60, 62 cm, du?ina trupa 67, 83 cm, ?irina prsa 17, 75 cm, dubina prsa 29, 34 cm, opseg prsa 83, 10 cm, opseg cjevanice 7, 93 cm i tjelesna masa 41, 58 kg. Utvr?eno je da dana?nje creske ovce imaju ve?i tjelesni okvir i tjelesnu masu u odnosu na ranija istra?ivanja (prije 50 i vi?e godina). ?ilje?ice su imale 93, 44% visine grebena, 94, 33% du?ine trupa, 92, 22% ?irine prsa, 89, 50% dubine prsa, 92, 19% opsega prsa, 97, 09% opsega cjevanice i 78, 86% tjelesne mase odraslih, tjelesno potpuno razvijenih creskih ovaca. Istra?ivanjem je utvr?eno da su creske ovce po tjelesnom okviru (razvijenosti) sli?ne li?koj pramenci i dubrova?koj ovci – rudi, donekle i pa?koj ovci, te znatno razvijenije od kr?ke ovce.
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Ostale indexne publikacije:
Chemical ACAB A AGRICOLA; AGRIS; Animal Breeding A Dairy Science Abstracts
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Znanstveni
Znanstvena podru?ja:
Agronomija
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Autor:Zhou YJ; Zhang ZS; Nie YQ; Cao J; Cao CY; Li YYEndere?o:Department of Gastroenterology and Hepatology, Guangzhou Digestive Diseases Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangdong, China.
Título:Association of adiponectin gene variation with progression of nonalcoholic fatty liver disease: A 4-year follow-up survey.
Fonte:J Dig D 16(10):601-9, 2015 Oct.
ISSN:País de publica??o:Australia
Idioma:ENGResumo:OBJECTIVE: To explore the role of tagging single nucleotide polymorphisms (tagSNPs) in the adiponectin gene in the natural course of nonalcoholic fatty liver disease (NAFLD). METHODS: The participants were chosen from our previous survey containing 3543 individuals. Finally, a total of 696 participants who had been followed up for a median of 4 years were included. Each participant was administered with an interview, physical examination, blood tests and ultrasonic examination at both baseline and end-point. Polymerase chain reaction-restriction fragment length polymorphism was applied to determine seven tagSNPs in the adiponectin gene, namely, rs182052, rs, rs822396, rs7627128, rs1501299, rs2241767 and rs3774261. Ordinal logistic regression was used to screen risk factors of NAFLD progression as well as the susceptibility to the disease. Haplotypes analyses were performed to confirm the results. RESULTS: After adjusting for age and gender, rs1501299 (G276T), rs2241767 (A45G) and rs3774261 (A712G) were found to be risk factors of both susceptibility (OR 5.040, 7.471 and 3.546, respectively) and progression (OR 3.83, 3.51 and 3.30, respectively) to NAFLD. Nevertheless, rs182052, rs, rs822396 and rs7627128 had no impact on them. These findings were confirmed by haplotype analysis. CONCLUSION: The tagSNPs rs2241767, rs1501299 and rs3774261 in the adiponectin gene are risk factors for the individuals' susceptibility to and progression of NAFLD.
Tipo de publica??o: JOURNAL ARTICLE
Nome de subst?ncia:0 (ADIPOQ protein, human); 0 (Adiponectin)
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Autor:Kim SH; Ezenwoye O; Cho HG; Robertson KD; Choi JHEndere?o:School of Computer Science and Engineering, Pusan National University, Busan, South Korea.
Título:iTagPlot: an accurate computation and interactive drawing tool for tag density plot.
Fonte:B 31(14):15 Jul 15.
ISSN:País de publica??o:England
Idioma:ENGResumo:MOTIVATION: Tag density plots are very important to intuitively reveal biological phenomena from capture-based sequencing data by visualizing the normalized read depth in a region. RESULTS: We have developed iTagPlot to compute tag density across functional features in parallel using multicores and a grid engine and to interactively explore it in a graphical user interface. It allows us to stratify features by defining groups based on biological function and measurement, summary statistics and unsupervised clustering. AVAILABILITY AND IMPLEMENTATION: http://sourceforge.net/projects/itagplot/.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
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Autor:Kumari A; Yadav SK; Misro MM; Ahmad J; Ali SEndere?o:Molecular Genetics Laboratory, National Institute of Immunology, New Delhi-110067, India.
Título:Copy number variation and microdeletions of the Y chromosome linked genes and loci across different categories of Indian infertile males.
Fonte:Sci R 5:1 Dec 07.
ISSN:País de publica??o:England
Idioma:ENGResumo:We analyzed 34 azoospermic (AZ), 43 oligospermic (OS), and 40 infertile males with normal spermiogram (INS) together with 55 normal fertile males (NFM) from the Indian population. AZ showed more microdeletions in the AZFa and AZFb regions whereas oligospermic ones showed more microdeletions in the AZFc region. Frequency of the AZF partial deletions was higher in males with spermatogenic impairments than in INS. Significantly, SRY, DAZ and BPY2 genes showed copy number variation across different categories of the patients and much reduced copies of the DYZ1 repeat arrays compared to that in normal fertile males. Likewise, INS showed microdeletions, sequence and copy number variation of several Y linked genes and loci. In the context of infertility, STS deletions and copy number variations both were statistically significant (p = 0.001). Thus, semen samples used during in vitro fertilization (IVF) and assisted reproductive technology (ART) must be assessed for the microdeletions of AZFa, b and c regions in addition to the affected genes reported herein. Present study is envisaged to be useful for DNA based diagnosis of different categories of the infertile males lending support to genetic counseling to the couples aspiring to avail assisted reproductive technologies.
Tipo de publica??o: JOURNAL ARTICLE
Nome de subst?ncia:0 (SRY protein, human); 0 (Sex-Determining Region Y Protein)
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Autor:Song L; Lu Y; Zhang J; Pan C; Yang X; Li X; Liu W; Li LEndere?o:National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Título:Physical mapping of Agropyron cristatum chromosome 6P using deletion lines in common wheat background.
Fonte:Theor Appl G 129(5):16 May.
ISSN:País de publica??o:Germany
Idioma:engResumo:KEY MESSAGE: Genetically stable deletion lines of Agropyron cristatum chromosome 6P in common wheat background were generated, which allowed for physical mapping of 255 6P-specific STS markers and leaf rust resistance gene(s). Chromosomal deletion lines are valuable tools for gene discovery and localization. The chromosome 6P of Agropyron cristatum (2n = 4x = 28, PPPP) confers many desirable agronomic traits to common wheat, such as higher grain number per spike, multiple fertile tiller number, and enhanced resistance to certain diseases. Although many elite genes from A. cristatum have been identified, their chromosomal locations were largely undetermined due to the lack of A. cristatum 6P deletion lines. In this study, various A. cristatum 6P deletion lines were developed using a wheat-A. cristatum 6P disomic addition line 4844-12 subjected to (60)Co-?? irradiation as well as an Aegilops cylindrica gametocidal chromosome. Twenty-six genetically stable A. cristatum 6P deletion lines in the genetic background of common wheat were obtained, and their genetic constitutions were elucidated by genomic in situ hybridization (GISH) and sequence-tagged site (STS) markers specific to A. cristatum chromosome 6P. Moreover, 255 novel chromosome 6P-specific STS markers were physically mapped to 14 regions of chromosome 6P. Field evaluation of leaf rust resistance of various deletion lines and BC1F2 populations indicated that the A.cristatum chromosome 6P-originated leaf rust resistance gene(s) was located in the region 6PS-0.81-1.00. This study will provide not only useful tools for characterization and utilization of wheat materials with alien chromosomal segments, but also novel wheat germplasms potentially valuable in wheat breeding and improvement.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de subst?ncia:0 (Genetic Markers)
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Autor:Kamel KA; Kroc M; Swiecicki WEndere?o:Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.
Título:Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.).
Fonte:Acta Biochim P 62(3):533-40, 2015.
ISSN:XPaís de publica??o:Poland
Idioma:engResumo:Sequence tagged site (STS) markers are valuable tools for genetic and physical mapping that can be successfully used in comparative analyses among related species. Current challenges for molecular markers genotyping in plants include the lack of fast, sensitive and inexpensive methods suitable for sequence variant detection. In contrast, high resolution melting (HRM) is a simple and high-throughput assay, which has been widely applied in sequence polymorphism identification as well as in the studies of genetic variability and genotyping. The present study is the first attempt to use the HRM analysis to genotype STS markers in narrow-leafed lupin (Lupinus angustifolius L.). The sensitivity and utility of this method was confirmed by the sequence polymorphism detection based on melting curve profiles in the parental genotypes and progeny of the narrow-leafed lupin mapping population. Application of different approaches, including amplicon size and a simulated heterozygote analysis, has allowed for successful genetic mapping of 16 new STS markers in the narrow-leafed lupin genome.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de subst?ncia:0 (DNA Primers); 0 (Genetic Markers)
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Autor:Leng P; Ji Q; Tao Y; Ibrahim R; Pan G; Xu M; Lübberstedt TEndere?o:National Maize Improvement Center, China Agricultural University, Beijing, 100094, C Department of Agronomy, Iowa State University, Ames, Iowa, 50011, United States of America....
Título:Characterization of Sugarcane Mosaic Virus Scmv1 and Scmv2 Resistance Regions by Regional Association Analysis in Maize.
Fonte:PLoS O 10(10):e15.
ISSN:País de publica??o:United States
Idioma:engResumo:Sugarcane Mosaic Virus (SCMV) causes one of the most severe virus diseases in maize worldwide, resulting in reduced grain and forage yield in susceptible cultivars. In this study, two association panels consisting of 94 inbred lines each, from China and the U.S., were characterized for resistance to two isolates: SCMV-Seehausen and SCMV-BJ. The population structure of both association panels was analyzed using 3072 single nucleotide polymorphism (SNP) markers. The Chinese and the U.S. panel were both subdivided into two sub-populations, the latter comprised of Stiff Stalk Synthetic (SS) lines and Non Stiff Stalk Synthetic (NSS). The relative kinships were calculated using informative 2947 SNPs with minor allele frequency ≥ 5% and missing data ≤ 20% for the Chinese panel and 2841 SNPs with the same characteristics were used for the U.S. panel. The Scmv1 region was genotyped using 7 single sequence repeat (SSR) and sequence-tagged site (STS) markers, and 12 SSR markers were used for the Scmv2 region in the U.S. panel, while 5 of them were used for the Chinese panel. For all traits, a MLM (Mix Linear Model) controlling both population structure and relative kinship (Q + K) was used for association analysis. Three markers Trx-1, STS-11, and STS-12 located in the Scmv1 region were strongly associated (P = 0.001) with SCMV resistance, and explained more than 16.0%, 10.6%, and 19.7% of phenotypic variation, respectively. 207FG003 located in the Scmv2 region was significantly associated (P = 0.001) with SCMV resistance, and explained around 18.5% of phenotypic variation.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
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Autor:Bernardo A; Wang S; St Amand P; Bai GEndere?o:Department of Plant Pathology, Kansas State University, Manhattan, Kansas, United States of America....
Título:Using Next Generation Sequencing for Multiplexed Trait-Linked Markers in Wheat.
Fonte:PLoS O 10(12):e15.
ISSN:País de publica??o:United States
Idioma:engResumo:With the advent of next generation sequencing (NGS) technologies, single nucleotide polymorphisms (SNPs) have become the major type of marker for genotyping in many crops. However, the availability of SNP markers for important traits of bread wheat (Triticum aestivum L.) that can be effectively used in marker-assisted selection (MAS) is still limited and SNP assays for MAS are usually uniplex. A shift from uniplex to multiplex assays will allow the simultaneous analysis of multiple markers and increase MAS efficiency. We designed 33 locus-specific markers from SNP or indel-based marker sequences that linked to 20 different quantitative trait loci (QTL) or genes of agronomic importance in wheat and analyzed the amplicon sequences using an Ion Torrent Proton Sequencer and a custom allele detection pipeline to determine the genotypes of 24 selected germplasm accessions. Among the 33 markers, 27 were successfully multiplexed and 23 had 100% SNP call rates. Results from analysis of "kompetitive allele-specific PCR" (KASP) and sequence tagged site (STS) markers developed from the same loci fully verified the genotype calls of 23 markers. The NGS-based multiplexed assay developed in this study is suitable for rapid and high-throughput screening of SNPs and some indel-based markers in wheat.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
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Autor:Zhang H; Zhang L; Wang C; Wang Y; Zhou X; Lv S; Liu X; Kang Z; Ji WEndere?o:State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China. zhangh1129@....
Título:Molecular mapping and marker development for the Triticum dicoccoides-derived stripe rust resistance gene YrSM139-1B in bread wheat cv. Shaanmai 139.
Fonte:Theor Appl G 129(2):369-76, 2016 Feb.
ISSN:País de publica??o:Germany
Idioma:engResumo:KEY MESSAGE: YrSM139-1B maybe a new gene for effective resistance to stripe rust and useful flanking markers for marker-assisted selection were developed. ABSTRACT: Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important foliar disease of wheat. Two dominant stripe rust resistant genes YrSM139-1B and YrSM139-2D were pyramided in bread wheat cultivar Shaanmai 139; one from wild emmer and the other from Thinopyrum intermedium. Three near-isogenic F7:8 line pairs (contrasting RILs), N122-1013R/S, N122-185R/S, and N122-1812R/S, independently derived from different F2 plants and differing at the YrSM139-1B locus were generated from the cross Shaanmai 139 × Hu 901-19 through marker-assisted selection. A large F2:3 population from cross N122-1013R × N122-1013S tested for stripe rust response and subjected to analysis with markers in the 1BS10-0.5 bin region using SSR expressed sequence tags (EST) and site-specific sequence markers developed from the 90 K Illumina iSelect SNP array. Five EST-STS markers and four allele-specific PCR markers were mapped to the YrSM139-1B region. The 30.5 cM genetic map for YrSM139-1B consisted of nine markers, two of which were closer to YrSM139-1B than Xgwm273, which was used in producing the contrasting RIL pairs. Race response data and allelism tests showed that YrSM139-1B is different from Yr10, Yr15, and Yr24/26/CH42.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de subst?ncia:0 (DNA, Plant); 0 (Genetic Markers)
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Autor:Kim SK; Min WK; Park YC; Seo JHEndere?o:Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea....
Título:Application of repeated aspartate tags to improving extracellular production of Escherichia coli L-asparaginase isozyme II.
Fonte:Enzyme Microb T 79-80:49-54, 2015 Nov.
ISSN:País de publica??o:United States
Idioma:engResumo:Asparaginase isozyme II from Escherichia coli is a popular enzyme that has been used as a therapeutic agent against acute lymphoblastic leukemia. Here, fusion tag systems consisting of the pelB signal sequence and various lengths of repeated aspartate tags were devised to highly express and to release active asparaginase isozyme II extracellularly in E. coli. Among several constructs, recombinant asparaginase isozyme II fused with the pelB signal sequence and five aspartate tag was secreted efficiently into culture medium at 34.6 U/mg cell of specific activity. By batch fermentation, recombinant E. coli produced 40.8 U/ml asparaginase isozyme II in the medium. In addition, deletion of the gspDE gene reduced extracellular production of asparaginase isozyme II, indicating that secretion of recombinant asparaginase isozyme II was partially ascribed to the recognition by the general secretion machinery. This tag system composed of the pelB signal peptide, and repeated aspartates can be applied to extracellular production of other recombinant proteins.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de subst?ncia:0 (Culture Media); 0 (Escherichia coli Proteins); 0 (Isoenzymes); 0 (Recombinant Fusion Proteins); 30KYC7MIAI (Aspartic Acid); EC 3.5.1.1 (Asparaginase)
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Autor:Ali L; Deokar A; Caballo C; Tar'an B; Gil J; Chen W; Millan T; Rubio JEndere?o:Department of Genetics, University of Córdoba, Campus Rabanales Ed. C-5, 14071, Córdoba, Spain....
Título:Fine mapping for double podding gene in chickpea.
Fonte:Theor Appl G 129(1):77-86, 2016 Jan.
ISSN:País de publica??o:Germany
Idioma:engResumo:KEY MESSAGE: For the first time, fine mapping for sfl locus was carried out using a battery of new STMS and SNP markers. The target region was delimited to 92.6 Kb where seven annotated genes were found that could be candidate genes for the simple/double podding trait in chickpea. Four recombinant inbred populations (RIP-1, RIP-7, RIP-11, and CPR-01) were used to map the double podding gene (sfl) in chickpea. In RIP-1, the gene was initially mapped on linkage group (LG) 6 between the two sequence-tagged microsatellite site (STMS) markers TA120 and TR1. Eight new STMS markers were added onto LG6 in the target region and sfl locus was finally located between CAGM27819 and CAGM27777 markers within an interval of 2 cM. Seven out of the eight markers were mapped in RIP-7 and its reciprocal RIP-11 confirming the location of the sfl locus to a 4.8 cM interval flanked by TR44 and CAGM27705. Furthermore, using a high-density single nucleotide polymorphism (SNP) map of CPR-01, sfl was mapped to the same genomic region in a 5.1 cM interval between TR44 and the SNP scaffold. Five pairs of near isogenic lines (NILs) and eight recombinant inbred lines (RILs) were used to refine this region in the chickpea physical map. Combining data from linkage analysis in four RIPs, marker physical positions and recombination events obtained in both pairs of NILs and selected RILs, sfl could be placed within a genomic window of 92.6 Kb. Seven annotated genes were extracted from this region. The regulator of axillary meristem-predicted gene could be a candidate gene for the simple/double podding gene. This study provides additional set of markers flanking and tightly linked to sfl locus that are useful for marker-assisted selection.
Tipo de publica??o: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nome de subst?ncia:0 (Genetic Markers)
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