Genetic diagnosis of Down's syndrome using the STR of 21 chromosome--《Chinese Journal of Child Health Care》2009年05期
Genetic diagnosis of Down's syndrome using the STR of 21 chromosome
WU Jin-fang1,LUO Li2,LIU Yu-xin3,MA Xiao-ping4,LIU Qi1,YANG Hua1.(1 Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Medical College,Xi'an Jiaotong University,Xi'an,Shaanxi
The Fourth People's Hospital of Xi'an,Xi'an,Shaanxi
Xi'an Jiaotong University,School of Life Science and Technology,Xi'an,Shaanxi
Shaanxi Maternal and Child Health Care Hospital,Xi'an,Shaanxi 710004,China)
【Objective】 To discuss the feasibility of genetic diagnosis of Down's syndrome using the STR of 21 chromosome and establish a rapid,accurate and fast way diagnosing the Down's syndrome. 【Methods】 The DNA samples of 18 Down's syndrome were amplified by PCR and polyacrylamide gel electrophoresis.The diagnosis was made by analyzing allelic distribution at 3 STR locus(D21S2052、D21S2054、D21S1446).In contrary to the karyotype analysis at the same time. 【Results】 All the 18 cases appeared three bands with ratio of 1∶1∶1 or two bands with ratio of 2∶1 in the sites of chosen STRs,which was coincident with the analysis of karyotype. 【Conclusion】 The method of PCR amplification of the three STR locus in the 21 chromosome is rapid,accurate and simple for genetic diagnosis of Down's syndrome.
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【CateGory Index】：
R725.9;R450
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(C)2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reservedDetermination of Ignition Loss of Sponge Palladium Product by Hydrogen Reduction Gravimetry--《Chinese Journal of Inorganic Analytical Chemistry》2014年02期
Determination of Ignition Loss of Sponge Palladium Product by Hydrogen Reduction Gravimetry
LIU WTAN WHAN SHE XLI YWU XLIN Bo;LUO XMA WZHU LSino-Platinum Metals Resources(Yimen)Co.Ltd.;State Key Laboratory of Advanced Technologies for Comprehensive Utilization of Platinum Metals,Sino-Platinum Metals Co.Ltd.;
Conditions for determination of ignition loss in sponge palladium product by hydrogen reduction gravimetry were systematically investigated.The effect of nonmetallic impurity content on analytical result was investigated.Our results were compared with those obtained by thermogravimetry.1~2g sample was weighted and the heating temperature was increased stepwise to 800 ℃.The ignition loss in sponge palladium were determined to be 0.2%,0.0302%and 0.0694% with the range(R),standard deviation(S),relative standard deviation(RSD,n=11)and repeatability limit(r)of ±0.0005% ~0.1%~0.%~5.2% and 0.0%,respectively.The ignition loss was mainly composed of oxygen and nitrogen,but charcoal content was neglectable.Analytical results using the proposed method were accurate,precise and in good agreement with those by thermogravimetry and can meet the requirement for analysis of 99.90%~99.99%sponge palladium product.
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【CateGory Index】：
TF836;O655.1
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HE Xiaotang,WU Xilong,HAN Shouli,LI Kun,TAN Wenjin, WANG Huan,LI Yong,LIU Wen,ZHENG Yun,DU Zhijie(Sino-Platinum Metals Resources Co.Ltd.,State Key Laboratory of Advanced Technologies for Comprehensive Utilization of Platinum Metals,Sino-Platinum Metals Co.Ltd.,Kunming 650106,China);[J];Precious M2012-04
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LI JJIANG Ling-LI Ji-LI CHAO Ting-WANG Peng-Tianjin Chemical Institute,CNOOC;;[J];Guangzhou Chemical I2014-03
LIU WHE XWANG HWU XLI YZHAO Yu;HAN SLI KLI HTAN MLI ZSino-Platinum Metals Resources (Yimen) Co. Ltd., State Key Laboratory of Advanced Technologies for Comprehensive Utilization of Platinum Metals,Sino-Platinum Metals Co. Ltd.;;[J];Precious M2014-01
HUANG YSHA JXU LLI WYU BZHAO YKunming Sino-Platinum Metals Catalyst Co. Ltd.;;[J];Precious M2014-01
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