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过表达质粒
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想研究MEG3过表达对于小鼠星型细胞的影响，怎样构建MEG3的过表达质粒？有公司可以直接给合成过表达质粒吗？还是要自己构建？刚开始接触分子生物学，哪位大神教我一下，不甚感激。
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请问楼主这个问题解决没？我也有类似困扰
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你如果手里有这个基因的相关质粒，可能可以直接用，或者PCR出来，扔到你需要的载体里去，很方便。 如果你手里没有这个质粒的话，那么，或者你做逆转录-PCR，把这个基因从mRNA里抠出来，再放到你要的质粒里，或者你知道这个基因的序列，交给公司合成，并且帮你扔到你要的载体里。一般基因合成有点小贵了，长的基因合成起来更贵。。
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kangaroo0513
你如果手里有这个基因的相关质粒，可能可以直接用，或者PCR出来，扔到你需要的载体里去，很方便。
如果你手里没有这个质粒的话，那么，或者你做逆转录-PCR，把这个基因从mRNA里抠出来，再放到你要的质粒里，或者你知道这个基因的序列，交给公司合成，并且帮你扔到你要的载体里。一般基因合成有点小贵了，长的基因合成起来更贵。。
要是交给公司的话，有什么公司推荐么，谢谢
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&&8:00-11:30,13:00-17:00(工作日)&&&cho cells 在 肿瘤学 分类中
的翻译结果:
查询用时：0.18秒
&在分类学科中查询
&&&&EFFECT OF EXPRESSION OF EXOGENOUS PDGF-A CHAIN ON GROWTH AND TRANSFORMATION OF CHO CELLS
&&&&异源PDGF-A链表达对CHO细胞生长和转化的作用
&&&&Influence of microencapsulated CHO cells translated mIFN-γ on T_H1/T_H2 in tumor burden mice
&&&&微囊化转mIFN-γ基因CHO细胞皮下移植促进荷瘤小鼠T_H1T_H2的漂移
&&&&Expression of human IL-24 gene in CHO cells and its anti-tumor effect in vitro
&&&&人IL-24基因在CHO细胞中的表达及其抗肿瘤效应
&&&&Objective:With the eukaryotic expression vector pcDNA3.0-hIL-24 and the prokaryotic expression vector pET-21a(+)-hIL-24, we make them transfect CHO cells and BL-21 respectively and expressed high performance in them.
&&&&目的:研究hIL-24基因重组的真核表达质粒pcDNA3.0-hIL-24在CHO细胞中的表达产物和原核表达质粒pET-21a(+)-hIL-24在大肠杆菌中高效表达的产物经部分纯化、复性后的rhIL-24蛋白对胃癌细胞(SGC7901)及胃癌裸鼠模型的抗肿瘤效应及其分子机理。
&&&&Then construct the eukaryotic express vector by PCR using pcDNA3.0 as a template and transfect the CHO cells.
&&&&pcDNA3.0-hIL-24重组质粒,经鉴定后转染CHO细胞,然后测定CHO培养上清中的rhIL-24的生物学活性。
&&&&EFFECT OF EXPRESSION OF EXOGENOUS PDGF-A CHAIN ON GROWTH AND TRANSFORMATION OF CHO CELLS
&&&&异源PDGF-A链表达对CHO细胞生长和转化的作用
&&&&Influence of microencapsulated CHO cells translated mIFN-γ on T_H1/T_H2 in tumor burden mice
&&&&微囊化转mIFN-γ基因CHO细胞皮下移植促进荷瘤小鼠T_H1T_H2的漂移
&&&&Expression of human IL-24 gene in CHO cells and its anti-tumor effect in vitro
&&&&人IL-24基因在CHO细胞中的表达及其抗肿瘤效应
&&&&Objective:With the eukaryotic expression vector pcDNA3.0-hIL-24 and the prokaryotic expression vector pET-21a(+)-hIL-24, we make them transfect CHO cells and BL-21 respectively and expressed high performance in them.
&&&&目的:研究hIL-24基因重组的真核表达质粒pcDNA3.0-hIL-24在CHO细胞中的表达产物和原核表达质粒pET-21a(+)-hIL-24在大肠杆菌中高效表达的产物经部分纯化、复性后的rhIL-24蛋白对胃癌细胞(SGC7901)及胃癌裸鼠模型的抗肿瘤效应及其分子机理。
&&&&Then construct the eukaryotic express vector by PCR using pcDNA3.0 as a template and transfect the CHO cells.
&&&&pcDNA3.0-hIL-24重组质粒,经鉴定后转染CHO细胞,然后测定CHO培养上清中的rhIL-24的生物学活性。
&&&&Effects of microencapsulated CHO cells modified with maspin gene on the motility and adhesiveness of breast carcinoma cells Bcap37
&&&&微囊化Maspin基因修饰细胞对乳腺癌细胞Bcap37运动及黏附功能的影响
&&&&Construction of eukaryotic expression vector of CD80-IgG1 Fc fragment fusion protein and its expression in CHO cells
&&&&CD80-IgG1Fc段融合蛋白真核表达载体的构建及表达
&&&&As P185~(erbB2) overexpress also in ovarian carcinoma and concern with poor prognosis and drug resistance, we then prepared humanized McAb against P185~(erbB2) and high-level expressed in CHO cells for adjuvant therapy of ovarian carcinoma.
&&&&应用P185~(erbB2)过度表达的SKBR_3细胞免疫BALB／c小鼠，采用P185~(erbB2)胞外区重组蛋白以ELISA方法筛选杂交瘤阳性克隆，获得了10株单克隆抗体。
&&&&The CHO cells expressing the AGT Leu84Phe and Leu84Phe/ Ile143Val/ Lys178Arg variants were less resistant to BCNU
induced cytotoxicity. In contrast, there was no difference in the toxic response to BCNU between wild
type AGT and the other AGT variants including Ile143Val, Lys178Arg, Ser184Asn, and le143Val/Lys178Arg.
&&&&【结果】表达AGTLeu84Phe和Leu84Phe/Ile143Val/Lys178Arg突变体的细胞对BCNU的毒性作用抵抗力显著减弱,与此不同的是,其余突变体Ile143Val、Lys178Arg、Ser184Asn以及Ile143Val/Lys178Arg对BCNU毒性作用的抵抗性较野生型AGT无明显差异。
&&&&All CHO cells which stably expressed very high level of TEF-1δ e ncoded protein demonstrated relatively high expression of cell cycle controlling
g ene Cyclin D1(encoded 36 kDa protein)as compared with the vector control.
&&&&同样 ,4株高效稳定表达TEF - 1δ编码蛋白质的CHO系均具有相对较高的细胞周期调控基因CyclinD1(编码蛋白 36kDa)的表达。
查询“cho cells”译词为用户自定义的双语例句&&&&我想查看译文中含有：的双语例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。&&&&&&&&&&&& The method of combining culture of embryonic chicken heart fragment with some cells in double-layer agar-agar medium was used in this experiment. With this method, the author investigated the invading capacity of the hybrid cells formed by fusing normal CHO cells with malignant Eca-109 cells, and the author also studied the effect of hybrid cells on the inhibition of the in ading capacity of the malignant parental cells Eca-109 to embryonic chicken heart fragment. 本实验采用双层琼脂体外心肌和细胞共培养的方法,研究了恶性人体食管癌细胞(Eca-109)以及该细胞和正常中国地鼠卵巢细胞(CHO)的杂交细胞(109×CHO)的体外浸润能力。实验表明:Eca-109细胞体外有浸润鸡胚心肌的能力,并有破坏正常组织的行为;Eca-109和CHO的杂交细胞不但自身的浸润能力降低,而且对其恶性亲本细胞Eca-109的浸润能力也有抑制作用;而正常CHO细胞的自身融合细胞不影响恶性Eca-109细胞的浸胞的浸润能力。 The mutagenicity of the two kinds of dust samples(oxidized and sulfurized)collected from a tin mine in Yunnan Province was detected by the chromosomeaberration in vitro test with CHO cell and in vivo micronucleus test in mice.DMSO was used as solvent in chromosome aberration test. The concentration ofthe two dusts is 0.01ml/ml media respectively. The doses of micronucleus testin mice were 250,625 and 1250mg/kg for oxidized dust and 250, 1250 and2500mg/kg for sulfurized dust orally given. None with an interval... The mutagenicity of the two kinds of dust samples(oxidized and sulfurized)collected from a tin mine in Yunnan Province was detected by the chromosomeaberration in vitro test with CHO cell and in vivo micronucleus test in mice.DMSO was used as solvent in chromosome aberration test. The concentration ofthe two dusts is 0.01ml/ml media respectively. The doses of micronucleus testin mice were 250,625 and 1250mg/kg for oxidized dust and 250, 1250 and2500mg/kg for sulfurized dust orally given. None with an interval of 24h ofthe samples was mutagenic by the two tests.本文对两种云南锡矿(氧化矿和硫化矿)矿生进行CHO细胞染色体畸变试验和小鼠骨髓微核试验。试验结果:两种矿尘两种试验均获得阴性结果。 CHO cells were transfected with plasmidpSV_2-PDGF-A (containing human PDGF-AcDNA) by calcium phosphate method.Twen-ty transfected cell lines were obtained afterG418 selection.The selected 2 cell lines At_1and Aot7).with prominent changes in mor-phology and growth behaviour,showed tran-scription of PDGF-A chain mRNA muchhigher than CHO cells,strong fluorescentPDGF-specific reaction,appearing that PDGF-like proteins were synthesized in cytoplasmof these cells.At_1 and Aot7 cells... CHO cells were transfected with plasmidpSV_2-PDGF-A (containing human PDGF-AcDNA) by calcium phosphate method.Twen-ty transfected cell lines were obtained afterG418 selection.The selected 2 cell lines At_1and Aot7).with prominent changes in mor-phology and growth behaviour,showed tran-scription of PDGF-A chain mRNA muchhigher than CHO cells,strong fluorescentPDGF-specific reaction,appearing that PDGF-like proteins were synthesized in cytoplasmof these cells.At_1 and Aot7 cells not onlyhad increased growth rate,but also formedlarge colonies in soft agar and grew intofibrosarcomas in nude mice.These resultssuggested that the expression of exogenousPDGF-A gene might cause the uncontrolledgrowth and malignant transformation of CHOcells.用DNA磷酸钙盐沉淀方法把含人PDGF(血小板衍生生长因子)A链cDNA的表达质粒pSV_2neo-A转染CHO细胞(中国仓鼠卵巢细胞),然后经G 418(400-800 μg/ml)筛选分离20个转染细胞株。选出其中At_1和Aot7细胞株所进行的实验结果表明,这些细胞的形态和生长行为均发生明显的变化,PDGF-A链mRNA的表达水平比CHO细胞明显增高,胞质有强阳性的PDGF荧光反应,显示有PDGF样蛋白的合成。这些细胞不但生长速率加快,有高密度持续生长的特性,而且能在软琼脂培基上形成大集落和在裸鼠体内接种形成纤维肉瘤,提示外源PDGF-A链基因的表达有使CHO细胞生长失控和发生细胞恶性转化的作用。&nbsp&&&&&相关查询
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