A probe is a piece of DNA or RNA used to detect specific nucleic acid
sequences by hybridization (binding of two nucleic acid chains by base
pairing) . They are radioactively labeled so that the hybridized nucleic
acid can be identified by autoradiography.
The size of probes ranges from a few nucleotides to hundreds of kilobases.
Long probes are usually made by cloning. Originally they may be
double-stranded, but the working probes must be single-stranded. Short probes (oligonucleotide
probes) can be made by chemical synthesis. They are single-stranded.
Suppose we have known the sequence of a specific
gene in yeast and want to find its homologous gene in human, then we may use the
specific yeast gene as a probe to detect its homologous gene from the human
genomic library. On the other hand, if we know the conserved sequence in
the specific gene between yeast and human, we may use oligonucleotide probes
containing only the conserved sequence. Typically, an oligonucleotide
about 20 nucleotides long is sufficient to screen a library.
In some cases, we have known the partial sequence of a protein and want to detect
its gene in the library. Then we may synthesize oligonucleotide probes
based on the known peptide sequence. Since an amino acid may be encoded by
several DNA triplets, many different oligonucleotide probes are often needed.
Figure 9-B-2. The relationship
between a peptide and all possible DNA sequences. In this example, the peptide sequence Leu-Phe-Tyr-Met-His-Asp
corresponds to 96 (= 6 x 2 x 2 x 1 x 2 x 2) possible DNA sequences.
Once a particular DNA fragment is identified, it can be isolated and
amplified to determine its sequence. If we know the partial sequence of a
gene and want to determine its entire sequence, the probe should contain the
known sequence so that the detected DNA fragment may contain the gene of
Figure 9-B-3. Screening of a specific DNA
fragment. After recombinant λ virions form
plaques on the lawn of E. coli, the nitrocellulose filter (membrane) is
placed on the surface of the petri dish to pick up λ
phages from each plaque. Then, the filter is incubated in an alkaline
solution to disrupt the virions and release the encapsulated DNA, which is
subsequently denatured. Next, the probe is added to hybridize with the
target DNA fragment, whose position may be displayed by autoradiography.genomic library
[遗] 基因组文库
基因组文库
原生质体(Protoplasts)
肿瘤转移(Neoplasm Metastasis)
红霉素(Erythromycin)
基因组文库(Genomic Library)
基于361个网页-
英汉日科技词汇——G ... genomic correlation ==& 染色体组相关 genomic library ==& 基因组库 genotype ==& 基因型 ...
基于127个网页-
关键词：喜盐芽孢杆菌(Halobacillus)，基因文库，甘氨酸甜菜碱转运蛋白
[gap=8911]Key words：Halobacillus，Genomic library，Glycine betaine transporter
基于18个网页-
基因图书馆
基因科学家利用细菌及滤过性病毒建立各种生物的「 基因图书馆 （ Genomic library ），以解读生命，殊不知此等图书馆中所有创作的版权是属于上帝的，而将荣耀归与创造的主？正如图书馆的每一本书都有作者一般。
基于6个网页-
基因组表达文库
基因组文库
基因组DNA文库
小麦基因组tac文库
基因组BAC文库
基因组文库
小麦基因组可转化人工染色体文库
基因组基因文库的大小
基因组dna基因库
更多收起网络短语
- 引用次数：8
参考来源 - 极端嗜热厌氧纤维素分解菌2菌株基因文库的构建及其纤维素酶系内切葡聚糖酶（ED）基因片段的克隆
基因组文库
基因组文库
- 引用次数：2
To study the antibiotic biosynthesis gene, the genomic library from the S. tenebrarius H6 was established firstly.
为了研究其菌体内抗生素生物合成基因，首先建立该菌株的完整基因文库。
参考来源 - 黑暗链霉菌S.tenebrarius H6中与抗生素有关生物合成基因的克隆
- 引用次数：1
参考来源 - 兼性嗜热古地分枝杆菌X7C脱有机硫关键酶黄素还原酶的纯化和性质分析
基因组文库
&2,447,543篇论文数据，部分数据来源于
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA.
以上来源于:
genomic library
【遗传学】染色体基因组信息库
以上来源于：《21世纪大英汉词典》
Contamination of the genomic library with chloroplast clones was very low (0.2%).
该基因组文库中叶绿体基因的污染率低于0.2%；
The construction of a genomic library is essential to research on genome structure and function.
基因组文库是进行分子克隆和基因组结构与功能研究的基础。
A high quality of the genomic library and an efficient method for transformation are the basics for cloning the genes by phenotypic complementation.
特别是条件突变体（如温度敏感型突变体等），通过表型互补克隆相关基因是一种基本的方法。
$firstVoiceSent
- 来自原声例句
请问您想要如何调整此模块？
感谢您的反馈，我们会尽快进行适当修改！
请问您想要如何调整此模块？
感谢您的反馈，我们会尽快进行适当修改！Genomic and expression profiling identifies the B-cell associated tyrosine kinase Syk as a possible therapeutic target in mantle cell lymphoma - Rinaldi - 2005 - British Journal of Haematology - Wiley Online Library
Advertisement
Among B-cell lymphomas mantle cell lymphoma (MCL) has the worst prognosis. By using a combination of genomic and expression profiling (Affymetrix GeneChip Mapping 10k Xba131 and U133 set), we analysed 26 MCL samples to identify genes relevant to MCL pathogenesis and that could represent new therapeutic targets. Recurrent genomic deletions and gains were detected. Genes were identified as overexpressed in regions of DNA gain on 3q, 6p, 8q, 9q, 16p and 18q, including the cancer genes BCL2 and MYC. Among the transcripts with high correlation between DNA and RNA, we identified SYK, a tyrosine kinase involved in B-cell receptor signalling. SYK was amplified at DNA level, as validated by fluorescence in situ hybridisation (FISH) analysis, and overexpressed at both RNA and protein levels in the JeKo-1 cell line. Low-level amplification, with protein overexpression of Syk was demonstrated by FISH in a small subset of clinical samples. After treatment with low doses of the Syk inhibitor piceatannol, cell proliferation arrest and apoptosis were induced in the cell line overexpressing Syk, while cells expressing low levels of Syk were much less sensitive. A combination of genomic and expression profiling suggested Syk inhibition as a new therapeutic strategy to be explored in lymphomas.