Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb.) by a simple freezing method
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, 1991, Pages 243-248
Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb.) by a simple freezing method
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aAsabucho 1-5-23, Sapporo, Kitaku, 001, JapanbAkitsu Branch, Fruit Tree Research Station, Akitsu, Hiroshima 729-24, Japan
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Suspension cultured cells of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) was cryopreserved without using a programmable freezer and dimethyl sulfoxide (DMSO) as a cryoprotectant. The nucellar cells were cryoprotected with a mixture of 2 or 3 M glycerol and 0.4 M sucrose dissolved in Murashige-Tucker basal medium (MT) for 10 min at 25°C. A cell suspension of about 0.2 ml was loaded into a 0.5-ml trasparent straw and frozen spontaneously by placing straws in a freezer at -30°C for 20–30 min prior to direct immersion into LN. The average rate of survival after thawing, as evaluated by fluorescein diacetate and phenosafranin double staining, was about 90% of non-frozen controls. We also obtained a similar survival rate using a 1.8-ml cryotube when the cell suspension was held at -30° for 40–50 min. The revived cells resumed growth within 3 days and developed into plantlets via embryogenesis. This simple procedure for cryopreservation seems promising for cultured plant cells and meristems.
MT Murashige-T
NAA α-naphthalene acetic acid
Remember meTransient Freezing Behavior in Photophobic Responses of Euglena gracilis Investigated in a Microfluidic Device
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Plant and Cell Physiology
Transient Freezing Behavior in Photophobic Responses of Euglena gracilis Investigated in a Microfluidic Device
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(a) Temporal changes in the TMs of nine individual Euglena cells corresponding to the TM change shown in Fig. 1b. The plots have been shifted vertically to display them in a single graph, while preserving the relative intensities of the
plots. These nine cells were grouped into three types, A, B and C, according to their behaviors during the blue-light-off
period, as described in the text. (b) Typical traces of the three types of Euglena cells shown in (a), observed for the same period. Circles, type-A; triangle, type-B; squares, type-C; open symbols, blue-light-
filled symbols, blue-light-off. Scale bar = 500 um. Type-A and type-B cells were swimming during the blue-light-off phase
and tumbling with intermittent swimming during the on phase, whereas type-C cells drifted with rotational tumbling during
the blue-light-on phase and became motionless during the off phase.
10.1093/pcp/pcu101
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