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钙的测定(EDTA滴定法)
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钙的测定(EDTA滴定法)
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你可能喜欢取100ml某水样,在PH=10的缓冲溶液中铬黑T为指示剂用EDTA标准溶液滴 至终点,用去0.0取100ml某水样,在PH=10的缓冲溶液中铬黑T为指示剂用EDTA标准溶液滴至终点,用去0.01mol/l的EDTA标准溶液22.66ml（滴定Ca、Mg离子总量）另取相同水样,用NaoH调节PH为12,加钙指示剂,用0.01mol/lEDTA标准溶液滴定至终点（单滴定Ca离子）,用去EDTA标准溶液16.48ml,计算该水样的Ca、Mg离子含量（mg/l）
如果要计算,EDTA标准溶液的浓度必须是0.01000mol/L,计算过程如下：EDTA和所有金属离子生成1： 1的配合物,已知钙的原子量是40.08,镁的原子量是24.31钙离子含量=0.*40.08/（100/1000）=66.05mg/L镁离子含量=0.01000*（22.66-16.48）*24.31/（100/1000）=15.02mg/L
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扫描下载二维码常用缓冲液配制(缓冲液配制) - 仪器教程 - 生物秀
标题: 常用缓冲液配制(缓冲液配制)
摘要: [常用缓冲液配制(缓冲液配制)] Buffer Formula ( 2 %)
Always to try to prepare buffer solution
at the temperature and concentration before plani [关键词:缓冲液配制]……
Buffer Formula ( 2 %)
Always to try to prepare buffer solution
at the temperature and concentration before planing to use during
the experiment. Stock solutions are acceptable as long as pH
adjustment made after temperature and concentration adjustment.
Also good buffers, e.g., Tris and Phosphate, are stable for a long
time period.
10X TE (10:2)
1X 1L 10X 10mM Tris-HCl 8.9 g
Tris-HCl 5.3 g Tris-Base 2 mM Na3 7.4 g
pH 8 at 25 C. Mix and store at 4 C. Periodically dump the 1X TE
and make up the fresh 1X TE from 10X stock.
10X TE (10:1)
1X 1L 1X 1L 10X 10mM Tris-HCl
1.06 g Tris-HCl 10.6 g 0.39 g Tris-Base 3.94 g 1mM
Na2 0.475 g 4.75 g pH 8 at 25 C. Mix and store at 4 C.
10X TE (50:50)
1X 1L 10X 50 mM -HCl 44.4
-HCl 26.5 Tris-Base 50 mM Na3EDTA 186 g
Store at 4 C.
TE (25:10) 50 mM Glucose
1X 0.25 L 1X 25 mM Tris-HCl 0.55
g Tris-HCl 0.33 g Tris-Base 10 mM Na3EDTA 0.92
g 50 mM Glucose 2.25 g Store at 4 C.
50 mM Tris pH 8.0 10 mM EDTA
50 mM 1 M Tris pH 8.0 5 ml 1 mM
0.5 M EDTA 2 ml H2O up to 100 ml Store at 4 C.
1 M Tris pH 8.0, 1.5 M NaCl
1X 1L 1X 2L 1X 1M Tris-HCl 88.8 g
Tris-HCl 178 g 53.0 g Tris-Base 106 g 1.5 M NaCl
87.7 g 175 g Store at 25 C (room temperature is OK)
1 M Tris pH 7.4, 1.5 M NaCl
1X 1L 1X 2L 1X 1M Tris-HCl 132.2
g Tris-HCl 264.4 g 19.4 g Tris-Base 38.8 g 1.5 M
NaCl 87.7 g 175 g Store at 25 C (room temperature is OK)
0.5 M Tris pH 7.5, 1.5 M NaCl
Conc. 1 L 2L 3 L 0.5 M Tris
Tris-HCl 63.5 g 127.0 190.0 Tris-Base 11.8 g 23.6 g 35.4
g 1.5 M NaCl 87.6 g 175.0 g 262.8 g Store at 25 C
(room temperature is OK)
10 mM Tris pH 7.5, 15 mM NaCl
Conc. Stock Volume 10 mM 1 M Tris
pH 7.5 100 μl 15 mM 5 M NaCl 30 μl H2O to 10
ml Store at 4 C.
10X TBE Buffer (pH 8)
1X 1L 10X 4L 10X 89 mM Tris-Base
108 g 432 g 89 mM Boric acid 55 g 220 g 2 mM Na2EDTA
9.3 g 37.2 g EtBr (10mg/ml) 0.5 ml 2 ml Mix and
store at room temperature without EtBr, 4 C with EtBr in a brown
bottle. Use EtBr stock solution (10 mg EtBr/ml) when TBE is made.
50X TAE Buffer (pH 8)
1X 1L 50X 1 L 10X 40 mM
Tris-acetate 242 g Tris Base 48.4 g 57.1 ml acetic acid
11.4 ml 2 mM Na2EDTA 37 g 7.4 g EtBr (10mg/ml) 2.5
ml 0.5 ml Mix and store at room temperature without EtBr, 4
C with EtBr in a brown bottle. Use EtBr stock solution (10 mg
EtBr/ml) when TAE is made.
Cotton DNA Extraction buffer (pH 6)
Conc. Stock 1L 1X 100 mM
Na2citrate.2H2O 29.4 g Glucose 63 g 5 mM 0.5 M
Na2EDTA 10 ml Na2Diethyldithiocarbamic acid 10 g
PVP-40,000 MW 20 g BSA 10 g Adjust to pH 6.0 with
HCl. Store at 4 C.
RSB (Reaction Stop Buffer)
Conc. Stock Volume (ml) 10 mM 1 M
Tris pH 8.0 1.0 2 mM 0.5 M EDTA 0.4 0.2 % 20 % SDA
1.0 H2O 97.6 Store at room temperature.
STE (Sodium chloride and TE) pH 8.0
Conc. Stock 1X 1 L 10X 1L 10 mM
1M Tris pH 8.0 10.0 8.88 g Tris-HCl 5.3 g Tris-Base
1 mM 0.5 M EDTA 2.0 4.65 g Na2EDTA 100 mM 5 M NaCl 20.0
5.84 g NaCl H2O up to 1 L up to 1 L pH 8.0 at 25 C.
Store at 4 C.
1.5 M NaCl 0.5 N NaOH
1X 1L 1X 2L 1X 0.5 N NaOH 20.0 g
40 g 1.5 N NaCl 87.7 g 175 g Store at room
temperature.
0.2 N NaOH 0.6 M NaCl
Conc. 1L 2L 3L 4L 0.2 N NaOH 8.0
g 16.0 g 24.0 g 32.0 g 0.6 M NaCl 35.04 g 70.08 g 105.2 g
140.2 g Store at room temperature.
20X SSC (Adjust pH 7.0)
1X 1L 20X 2L 4L 6L 150 mM NaCl
175.3 g 350.6 g 701 g 1052 g 15 mM Na3Citrate 88.3 g 176.6
g 353 g 530 g Mix, adjust pH to 7.0 with HCl, and store at
25X SSC (Adjust pH 7.4)
25X 1L 4L 3.0 M NaCl 219 g 876
g 0.3 M Na2Citrate 110 g 440 g Mix, adjust pH to 7.4
with HCl, and store at 4C.
3 M K 5 M Ac
1X 200 ml 1 X 3 M KAc 29.4 g (or
20 ml of 5 M KAc) 2 M Acetic acid 23 ml Dissolve the
KAc in 150 ml of H2O, bring to 177 ml, and then add the glacial
acetic acid. Mix and store at 4 C.
Minimal Hybridization Buffer
Conc. Stock 1L 2L 10 % 50 % PEG
200 400 7 % 20 %
350 700 0.6 X 25 X SSC 24
48 10 mM 1 M NaHPO4 10 20 5 mM 0.5 M EDTA 10
20 100 μg/ml Denature Salmon Sperm 10 20 H2O 396
792 Aliquote 45 ml into 50 ml PPT, store at -20 C.
Oligo Buffer
After preparing the following stock
solutions, mix A, B, and C in a ratio of 1:2.5:1.5, repectively.
Solution A
Stock 2-mercaptoethanol (bME) 18
μl DXTPs (A, T, G) 5 μl each Solution O 850 μl
Store at -20 C
Solution O
Conc. Stock 250 ml 100 ml 1.47 M
Tris-Base 44.52 g 17.81 g 0.147 M MgCl2 7.47 g 2.99 g
Adjust pH to 8.0 with conc. HCl.
Solution B
: 2M hepes buffer Conc. Stock 250
ml 100 ml 2 M Hepes 119.15 g 47.66 g Adjust pH to
6.6 with 4 N NaOH. Store at 4 C.
Solution C
Hexamer or oligo nucleotides. Add 55 μl
H2O directly to the Pharmacia bottle which contains 50 units of
lyophilized hexamer. Store at -20 C.
Dilute to 1 unit Klewnow fragment by
adding klewnow buffer. Stock Klenow from BRL comes as 500 units in
84 μl. Dilute to 1 unit by adding 420 μl of klenow buffer to the
500 unit/84 μl stock. Store - 20 C.
Klenow buffer (250 ml)
Conc Stock 7 mM Tris-HCl 0.211
g 7 mM MgCl2 0.355 g 50 mM NaCl 0.730 g 50 %
Glycerol 125 ml Add the above to about 80 ml H2O. Stir to
dissolve. Adjust volume to 250 ml with H2O. Store at -20 C.
REact buffer (See Restriction enzyme
buffer formula)
10 X REact buffer 2 : for, e.g. Hind
III and Sst 1 (5 ml)
1 X Conc. Stock 500 mM 1 M Tris
(pH 8.0) 2.5 ml 100 mM 1 M MgCl2 0.5 ml 500 mM NaCl
0.5 ml 25 mM Spermidine(3HCl) 0.032 g H2O 1.5
ml Mix, aliquote 2 ml in screw-cap vial, store at -20 C.
10 X REact buffer 3 : for, e.g. Eco RI
1 X Conc Stock 500 mM 1M Tris (pH 8.0) 2.5
ml 100 mM 1M MgCl2 0.5 ml 1 M 5 M NaCl 1.0
ml 25 mM Spermidine(3HCl) 0.032 g H2O 1.0 ml
Mix, aliquote 2 ml in screw-cap vial, store at -20 C.
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