基于jQuery图像碎片切换效果插件FragmentFly
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基于jQuery图像碎片切换效果插件FragmentFly
基于jQuery图像碎片切换效果插件FragmentFly。这是一款只需三步轻松完成碎片动画，参数可调，使用方便。
部分代码：
&div class="all_wrap"&
&div class="wrap_head"&
&div id="fragment_title"&
&div class="wrap_middle"&
&div class="wrap_middle_head"&
&ul class="nav_ul"&
&li&&a href="#"&使用说明&/a& &/li&
&li&&a href="#"&配置说明&/a& &/li&
&li&&a href="#"&动画模拟&/a& &/li&
&li&&a href="#"&author&&span class="dot"&:&/span&&span class="ahkari"&&Ahkari&/span&&/a&
&div class="clearFloat"&
&div class="wrap_middle_body"&
&div class="warp_middle_body_wrap"&
&div class="parm_info"&
&div class="parm_info_title" id="useInformation"&
使用说明&/p&
&div class="infoArea"&
&p class="heigher"&
步骤一：html&/p&
创建运用背景图片的元素&/p&
&div class="codeArea"&
&pre class="brush:"&
&!-- 对fragment_title使用fragmenFly插件 --&
&div id="fragment_title"&
&p class="heigher"&
步骤二：css&/p&
&&&&1.&&该元素设为相对定位，便于分割后的子元素进行定位。&/p&
&&&&2.&&宽高设为与被分割的图片一致，可确保精准分割。&/p&
&div class="codeArea"&
&pre class="brush:"&
#fragment_title{
/*必须设为relative*/
/*宽高与被分割的背景图片一致*/
width: 424
height:150
&p class="heigher"&
步骤三：javascript&/p&
&&&&1.&&通过jquery实现，需要导入所需文件。&/p&
&div class="codeArea"&
&pre class="brush:"&
&!-- 导入jquery或有jquery环境 --&
&script src="../libs/jquery.js" type="text/javascript"&&/script&
&!-- 导入插件 --&
&script src="../jquery.fragmentFly.js" type="text/javascript"&&/script&
&&&&2.&&对元素运用插件，参数设置除了图片目录都有默认值。&/p&
&div class="codeArea"&
&pre class="brush:"&
/*对背景元素使用插件方法*/
$("#fragment_title").fragmentFly({
image_url:"./img/title.png",
//背景图路径，当前目录为元素所在的html目录
cut_dir:"x",
//可选"x"或"y"，默认均分x方向
ave_part:12,
//均分cut_dir方向，默认切割成12份
rm_part:[2,3]
//非cut_dir方向上随机切割，默认最小2份，最多3份
anime_dir:"down",
//切割子元素动画运行方向，可选"up","down","left","right"，默认为down
path:[500,800],
//切割子元素动画路长，默认路径最短500px，最长800px
//切割子元素动画时长，默认时长最短1000ms，最长1300ms
opacity:[1,1]
//切割子元素透明度变化，默认初始为1，结束为1(即无渐变)
&&&&&&快速配置如下。&/p&
&div class="codeArea"&
&pre class="brush:"&
/*快速配置*/
$("#fragment_title").fragmentFly({image_url:"./img/title.png"},{});
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Ma et al . Virology Journal /content/8/1/315
Table 3Genotypes of all 50HBV DNA-positive cases
Genotype Total patients CHB ACHB P a
value Genotype B 11(22%)7(21%)4(24%)0.629Genotype C 25(50%)18(55%)7(41%)0.370Genotype D 14(28%)8(24%)6(35%)0.410
0.3BC 0.004BC 0.005BC 0.271CD 0.024
ACHB:asymptomatic carriers of HBV; CHB:chronic hepatitis B; BC:P value between genotypes B and C; BD:P value between genotypes B and D; CD:P value between genotypes C and D; P a :P value between patients with chronic hepatitis B and HBV asymptomatic carriers.
HBV genome have been developed, including restriction fragment length polymorphism [18],multiplex PCR with type-specific primers [19-21],and others. However, most of these newer methods are based on analyzing the S gene. Here we have developed a novel genotyping method based on a segment of the HBV DNA polymerase gene. Using CLUSTAL V, we demonstrated that the eight standard genotypes selected from the GenBank (NCBI)had interge-notypic divergence of at least 8%in their complete nucleo-tide sequences [2-7].We then confirmed that the semi-nested PCR products from the DNA polymerase gene had intergenotypic divergence of at least 4%,which is in accor-dance with the HBV DNA S gene sequence [8],except between genotypes A and G, B and G, and F and H. How-ever, the intergenotypic divergence among genotypes A through F were higher than 4%in our selected region. Genotypes F and H have only been detected in Central and South America, and genotype G has been identified in France, Germany, Mexico, and the United States. Geno-type A is more prevalent in northwestern Europe, North America, India, and sub-Saharan Africa. Only genotypes B, C, D, and A have been found in China [22-30].There-fore, the fragment of HBV DNA polymerase gene can be used for genotyping hepatitis B in China. This genotyping method can also be used to predict antiviral therapeutic response among HBV genotypes and the development of drug resistant due to mutations. It is a valuable tool for guiding the treatment of lamivudine-resistant HBV in the clinical setting [27-30].
We analyzed the nucleotide sequences of the semi-nested PCR products of the HBV DNA polymerase gene in the 50patient samples using CLUSTAL V. Genotypes C, B and D were detected while genotypes A, E, F, G, and H were not. Half of the HBV DNA polymerase-positive samples were genotype C, making it the domi-nant genotype. It was also the major genotype (55%)in the 33patients with chronic hepatitis B. The differences in the proportions of genotypes B, C, and D were not significant in the 17asymptomatic carriers of HBV. The proportion of genotype D was similar to genotype B, and its proportion in asymptomatic carriers of HBV was slightly, but not significantly (p=0.410), higher than
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that in patients with chronic hepatitis B. Our results suggest that genotype C is the dominant genotype among asymptomatic carriers and that genotype D may be more frequent in asymptomatic carriers than in patients with chronic hepatitis B.
Conclusions
A new method of genotyping HBV via sequencing a frag-ment of its DNA polymerase gene is a valid strategy for genotyping hepatitis B in areas with a high prevalence of genotypes A through F. It provides a novel alternative to complete sequencing of the HBV genome and allows the study of the relationship between genotype and muta-tions of HBV DNA polymerase gene induced by antiviral therapy. Using this method, genotypes C, B, and D were identified in patients from Shenyang, China, and geno-type C was demonstrated to be the dominant genotype. However, analysis of the difference in the proportions of the genotypes between asymptomatic carriers of HBV and patients with chronic hepatitis B, as well as the impact of genotype on therapeutic response and virus mutation, require further study.
List of abbreviations
HBV:hepatitis B PCR:polym HCC:hepatocellular carcinoma.
Acknowledgements
We would like to thank the support of the laboratory of Department of
Infectious Disease, Shengjing Hospital of China Medical University. This study was supported by China National Fund of Ministry of Science and
Technology (),Liaoning Provincial Fund of Provincial Department of Science and Technology (-7).And this study was also
supported by Shenyang Science and Technology Program No. F10-205-1-10. Author details 1
Department of Neurology, Shengjing Hospital of China Medical University, Shenyang 110817, China. 2Department of Infectious Disease, Shengjing Hospital of China Medical University, Shenyang 110817, China.
Authors ’contributions
YM and XGD designed, executed and coordinated the study. YM, YD and XGD contributed in the sample acquirement and laboratory analysis. YM, JF and XGD participated in the drafting of the manuscript and literature search. All authors read and approved the final manuscript. Competing interests
The authors declare that they have no competing interests. Received:24March 2011Accepted:22June 2011Published:22June 2011
References
1. Lai CL, Ratziu V, Yuen MF, Poynard T:Viral hepatitis B. Lancet 2003,
2. Okamoto H, Tsuda F, Sakugawa H, Sastrosoewignjo RI, Imai M, Miyakawa Y,
Mayumi M:Typing hepatitis B virus by homology in nucleotide sequence:comparison of surface antigen subtypes. J Gen Virol 5-2583.
3. Norder H, CouroucéAM, Magnius LO:Complete genomes, phylogenetic
relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 9-503.
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