Photo-Fermentative Hydrogen Production by Rhodobacter Sphaeroides KD131 under Various Culture Conditions | Korea Science
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Photo-Fermentative Hydrogen Production by Rhodobacter Sphaeroides KD131 under Various Culture Conditions
&Title & Authors
Photo-Fermentative Hydrogen Production by Rhodobacter Sphaeroides KD131 under Various Culture Conditions
Son, Han-Na; Kim, Dong-H Lee, Won-T Rhee, Young-Ha; Kim, Mi-S
Purple non-sulfur (PNS) bacterium
KD131 was studied with the aim of achieving maximum hydrogen production using various carbon and nitrogen sources at different pH conditions. Cells grew well and produced hydrogen using
or glutamate as a nitrogen source in combination with a carbon substrate, succinate or malate. During 48h of photo-heterotrophic fermentation under 110 illumination using a halogen lamp at , 67% of 30mM succinate added was degraded and the hydrogen yield was estimated as 3.29mol /mol-succinate. However, less than 30% of formate was consumed and hydrogen was not produced due to a lack of genes coding for the formate-hydrogen lyase complex of strain KD131. Initial cell concentrations of more than 0.6g dry cell weight/L-culture broth were not favorable for hydrogen evolution by cell aggregation, thus leading to substrate and light unavailability. In a modified Sistrom's medium containing 30mM succinate with a carbon to nitrogen ratio of 12.85 (w/w), glutamate produced 1.40-fold more hydrogen compared to ammonium sulfate during the first 48h. However, ammonium sulfate was 1.78-fold more effective for extended cultivation of 96h. An initial pH range from 6.0 to 9.0 influenced cell growth and hydrogen production, and maintenance of pH 7.5 during photofermentation led to the increased hydrogen yield.
BRhodobacter sphaeroides KD131;Photo-PNS
&References
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worse I'm afraid Bill isn't at home now.Can l take message?Andy puts the chair in the front of the door.I have two brothers.One is now in China and the other is in Canada
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